PRIMER_TM_FORMULA=0
PRIMER_SALT_CORRECTIONS=0
PRIMER_LOWERCASE_MASKING=0
PRIMER_NUM_RETURN=1
PRIMER_TASK=pick_left_only
PRIMER_EXPLAIN_FLAG=1
PRIMER_MIN_SIZE=10
PRIMER_MAX_SIZE=10
PRIMER_OPT_SIZE=10
PRIMER_MIN_TM=0
PRIMER_MAX_TM=70
P3_COMMENT=nomasking_Breslauer_Schildkraut
SEQUENCE_ID=000
SEQUENCE_TEMPLATE=TCCTTGTGAT
=
PRIMER_TM_FORMULA=0
PRIMER_SALT_CORRECTIONS=1
PRIMER_LOWERCASE_MASKING=0
P3_COMMENT=nomasking_Breslauer_SantaLucia
SEQUENCE_ID=001
SEQUENCE_TEMPLATE=TCCTTGTGAT
=
PRIMER_TM_FORMULA=0
PRIMER_SALT_CORRECTIONS=2
PRIMER_LOWERCASE_MASKING=0
P3_COMMENT=nomasking_Breslauer_Owczarzy
SEQUENCE_ID=002
SEQUENCE_TEMPLATE=TCCTTGTGAT
=
PRIMER_TM_FORMULA=0
PRIMER_SALT_CORRECTIONS=0
PRIMER_LOWERCASE_MASKING=1
P3_COMMENT=masking_Breslauer_Schildkraut
P3_COMMENT=Should fail
SEQUENCE_ID=100
SEQUENCE_TEMPLATE=TCCTTGTGAt
=
PRIMER_TM_FORMULA=0
PRIMER_SALT_CORRECTIONS=1
PRIMER_LOWERCASE_MASKING=1
SEQUENCE_ID=101
P3_COMMENT=masking_Breslauer_SantaLucia
P3_COMMENT=Should succeed
SEQUENCE_TEMPLATE=TCCTTGTGaT
=
PRIMER_TM_FORMULA=0
PRIMER_SALT_CORRECTIONS=2
PRIMER_LOWERCASE_MASKING=0
P3_COMMENT=masking_Breslauer_Owczarzy
P3_COMMENT=Should succeed
SEQUENCE_ID=102
SEQUENCE_TEMPLATE=tccttgtgat
=
PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=0
PRIMER_LOWERCASE_MASKING=0
P3_COMMENT=nomasking_SantaLucia_Schildkraut
SEQUENCE_ID=010
SEQUENCE_TEMPLATE=TCCTTGTGAT
=
PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_LOWERCASE_MASKING=0
P3_COMMENT=nomasking_SantaLucia_SantaLucia
SEQUENCE_ID=011
SEQUENCE_TEMPLATE=TCCTTGTGAG
=
PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=2
PRIMER_LOWERCASE_MASKING=0
P3_COMMENT=nomasking_SantaLucia_Owczarzy
SEQUENCE_ID=012
SEQUENCE_TEMPLATE=GCCTTGTGAG
=
PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=0
PRIMER_LOWERCASE_MASKING=1
P3_COMMENT=masking_SantaLucia_Schildkraut
P3_COMMENT=Should fail
SEQUENCE_ID=110
SEQUENCE_TEMPLATE=TCCTTGTGAt
=
PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=1
PRIMER_LOWERCASE_MASKING=1
P3_COMMENT=masking_SantaLucia_SantaLucia
P3_COMMENT=Should succeed
SEQUENCE_ID=111
SEQUENCE_TEMPLATE=TCCTTGTGaT
=
PRIMER_TM_FORMULA=1
PRIMER_SALT_CORRECTIONS=2
PRIMER_LOWERCASE_MASKING=0
P3_COMMENT=nomasking_SantaLucia_Owczarzy
P3_COMMENT=Should succeed
SEQUENCE_ID=112
SEQUENCE_TEMPLATE=tccttgtgat
=
SEQUENCE_ID=masking_001
PRIMER_LOWERCASE_MASKING=1
PRIMER_PRODUCT_SIZE_RANGE=40-300
PRIMER_TASK=pick_pcr_primers
PRIMER_MAX_SIZE=20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=20
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCTGGGTACTAAT
=
SEQUENCE_ID=masking_002
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNaATCACACCTGGGTACTAAT
=
SEQUENCE_ID=masking_003
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCTGGGTACTAAt
=
SEQUENCE_ID=masking_004
SEQUENCE_TEMPLATE=gCCTTCATTAGAAAGTGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCTGGGTACTAAT
=
SEQUENCE_ID=masking_005
SEQUENCE_TEMPLATE=GCCTTCATTAGAAAGTGACaNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCACACCTGGGTACTAAT
=
